- 1). Wipe down the micropropagating area and any tools with 1/4 cup of household bleach mixed with a gallon of water. This sterilizes the area.
- 2). Mix 1/8 cup granulated sugar, 1 cup distilled water, 1 cup liquid fertilizer mixture (1/4 tablespoon of 10-10-10 fertilizer mixed with 1 gallon of water), 1/2 Inositol tablet, 1/4 vitamin tablet with thiamine, and 2 tablespoons of agar flakes in a sterilized 2-quart pot. This recipe makes one pint of growing medium.
- 3). Place the pot on the stove and stir constantly while bringing the mixture to a boil. Cook until sugar has dissolved. Fill four small canning jars with 1 inch of growing medium. Loosely screw on the lids. Put the jars into a pot of boiling water and sterilize for 30 minutes. Place the jars aside to cool.
- 4). Cut four stems from a disease-free acacia plant 1/2 inch above and 1/2 inch below a leaf node, using garden shears. Strip the bark off the stems except for the area around leaf node. Dip the specimen in the bleach solution. Remove stem and cutoff any part damaged by the bleach.
- 5). Place the acacia stems in a jar with growing medium. Place the jar in a controlled environment such as a greenhouse. The cells in the stem will start to develop into an acacia plant within a few weeks.
- 6). Transplant the plant into a flowerpot filled with sterile potting soil when a full set of roots have developed. Keep in a controlled environment until ready to plant outside.
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