How Long Can Tobacco Smoke be Detected with a Nicotine & Metabolite Serum Test?

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    Average Nicotine Levels

    • In active tobacco users, average nicotine levels range from 30 ng/mL (nanograms per milliliter) to 50 ng/mL. Nicotine is metabolized by the liver to produce the chemical cotinine (an anagram of nicotine). While nicotine has a short half-life, cotinine is metabolized in the system at a much slower rate.

    Testing

    • A nicotine and nicotine metabolite test measures nicotine and its metabolite cotinine in the system. It is with both these values that determination of nicotine usage can be made.

    Measurements and Time

    • In heavy tobacco users, nicotine and cotinine levels in serum reduce after two weeks of abstinence to less than 2 ng/mL. This measurement is the standard in determining non-usage. Levels higher than 2 ng/mL indicate the test subject might be continuing to use tobacco or has been exposed to secondhand smoke.

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